General Stability | Organism |
---|---|
2-mercaptoethanol stabilizes during dialysis and heating | Rattus norvegicus |
24 h dialysis against 35 mM potassium phosphate buffer, pH 7.4, with addition of 5 mM 2-mercaptoethanol or dithiothreitol, stable | Rattus norvegicus |
irreversible denaturation of enzyme in dilute solutions below 1 mg/ml | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0018 | - |
Uracil | - |
Rattus norvegicus | |
0.0026 | - |
thymine | - |
Rattus norvegicus | |
0.0029 | - |
NADP+ | in presence of dihydrouracil | Rattus norvegicus | |
0.0038 | - |
NADP+ | in presence of dihydrothymine | Rattus norvegicus | |
0.011 | - |
NADPH | in presence of uracil | Rattus norvegicus | |
0.015 | - |
NADPH | in presence of thymine | Rattus norvegicus | |
0.043 | - |
Dihydrothymine | - |
Rattus norvegicus | |
0.193 | - |
dihydrouracil | - |
Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | 1 mol of enzyme contains 3 mol of iron | Rattus norvegicus | |
Iron | iron atoms are probably present in iron-sulfur centers | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
110000 | - |
2 * 110000, SDS-PAGE | Rattus norvegicus |
220000 | - |
gel filtration, sedimentation equilibrium | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
thymine + NADPH | Rattus norvegicus | rate-limiting enzyme of thymidine and uridine degradation | dihydrothymine + NADP+ | - |
r | |
uracil + NADPH | Rattus norvegicus | - |
5,6-dihydrouracil + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.7 | - |
substrate nitouracil | Rattus norvegicus |
20.9 | - |
substrate 5-iodouracil | Rattus norvegicus |
21.5 | - |
substrate 5-diazouracil | Rattus norvegicus |
27.9 | - |
substrate thymine | Rattus norvegicus |
34.9 | - |
substrate uracil | Rattus norvegicus |
46.9 | - |
substrate 5-bromouracil | Rattus norvegicus |
47.4 | - |
substrate 5-fluorouracil | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,6-dihydrouracil + NADP+ | - |
Rattus norvegicus | uracil + NADPH | - |
r | |
5-bromouracil + NADPH | - |
Rattus norvegicus | 5-bromo-5,6-dihydrouracil + NADP+ | - |
? | |
5-diazouracil + NADPH | - |
Rattus norvegicus | ? | - |
? | |
5-fluorouracil + NADPH + H+ | - |
Rattus norvegicus | 5-fluoro-5,6-dihydrouracil + NADP+ | - |
? | |
5-iodouracil + NADPH | - |
Rattus norvegicus | 5-iodo-5,6-dihydrouracil + NADP+ | - |
? | |
5-nitrouracil + NADPH | - |
Rattus norvegicus | ? | - |
? | |
dihydrothymine + NADP+ | - |
Rattus norvegicus | thymine + NADPH | - |
r | |
thymine + NADPH | - |
Rattus norvegicus | dihydrothymine + NADP+ | - |
r | |
thymine + NADPH | rate-limiting enzyme of thymidine and uridine degradation | Rattus norvegicus | dihydrothymine + NADP+ | - |
r | |
uracil + NADPH | - |
Rattus norvegicus | 5,6-dihydrouracil + NADP+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 110000, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
t1/2: 2 min without 2-mercaptoethanol, 2.5 min with 5 mM 2-mercaptoethanol | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
- |
Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
flavin | enzyme contains 4 mol of FAD per mol of enzyme | Rattus norvegicus | |
NADP+ | - |
Rattus norvegicus | |
NADPH | - |
Rattus norvegicus |